SIGMA SIGMA| 人血清白蛋白
- 公司名稱 沃卡威（北京）生物技術(shù)有限公司
- 品牌
- 型號 SIGMA
- 產(chǎn)地 進(jìn)口
- 廠商性質(zhì) 代理商
- 更新時間 2016/7/12 11:03:13
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主要產(chǎn)品有抗原抗體|無血清培養(yǎng)基|細(xì)胞培養(yǎng)耗材并代理銷售Thermo、Abcam、Armosco、Invitrogen(Gibco)、Roche、Sigma、R&D、ATCC、HYCLONE、Meridian等二十多家國外。
ALBUMIN, HUMAN
The source material for all human albumins made by Sigma has tested negative for HIV and HBSAG.
Sigma tests the solubility of human albumin powders in water at 50 mg/. In the experience of Sigma production chemists, aqueous aliquots stored at –20 °C are stable for several months. Repeated freezing and thawing of solutions is not recommended.
| A9511 | Albumin from human serum, lyophilized powder, ≥97% (agarose gel electrophoresis), | H2O: soluble50 mg/mL | ≥97% (agarose gel electrophoresis) | Sigma |
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| A1653 | Albumin from human serum, lyophilized powder, ≥96% (agarose gel electrophoresis), | H2O: soluble50 mg/mL | ≥96% (agarose gel electrophoresis) | Sigma |
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| A3782 | Albumin from human serum, lyophilized powder, Fatty acid free, Globulin free, ≥99% (agarose gel electrophoresis), | H2O: soluble50 mg/mL | ≥99% (agarose gel electrophoresis) | Sigma |
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| A8763 | Albumin from human serum, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis), | H2O: soluble20 mg/mL | ≥99% (agarose gel electrophoresis) | Sigma |
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| A1887 | Albumin from human serum, lyophilized powder, essentially fatty acid free, | H2O: soluble50 mg/mL, clear to slightly hazy, yellow | Sigma |
| |
| A4327 | Albumin from human serum, lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis), | H2O: soluble50 mg/mL | ≥96% (agarose gel electrophoresis) | Sigma |
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| A5843 | Albumin from human serum, lyophilized powder, low endotoxin, | H2O: soluble50 mg/mL | Sigma |
| |
| 05420 | Albumin from human serum, lyophilized powder, ≥95.0% (agarose gel electrophoresis), | ≥95.0% (agarose gel electrophoresis) | Sigma |
PRODUCTNO. |
PREPARATION/PURIFICATION |
SPECIAL CHARACTERISTICS |
A 1653 | Fraction V1 From human serum. |
Powder 96-99% by agarose gel (remainder mostly globulins) |
A 2817 | Fraction V1 |
Powder 96-99% by agarose gel (remainder mostly globulins) Produced specifically for bulk usage. |
A 1887 | Fraction V1, heat step2 Prepared from A 1653. |
Powder ³96% by agarose gel Essentially fatty acid free (∼0.005%) |
A 9511 |
1× crystallized and lyophilized. Prepared from A 1653 using method IV of Cohn, et al.3 |
Powder 97-99% by agarose gel |
A 8763 | Prepared from A 1653.4 |
Powder Essentially globulin free |
A 3782 | Prepared from A 8763.4 |
Powder Essentially fatty acid free (∼0.005%) Essentially globulin free |
A 4327 |
Prepared from A 1653. |
Powder ³96% by agarose gel Essentially protease free (<0.0001 u/mg protein) Essentially alkaline phosphatase free (0.001 u/mg protein) Essentially peroxidase free (<0.001 u/mg protein) |
A 3173 |
Prepared from A 2817. |
Powder Low endotoxin content (²0.1 ng/mg protein) |
A 6784 |
Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled. |
10% Solution |
A 6909 |
Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled. |
30% Solution |
A 9080 |
From human plasma. Contains approx. 0.85% NaCl. Aseptically filled. |
30% Solution Protease free. |
A 2830 |
From human serum. |
Solution in 40 mM potassium phosphate, pH 7.0 14C-Methylated 5-50 ìCi per mg protein |
CAS NUMBER: 70024-90-7
MOLECULAR WEIGHT: 66,248.35 or 66,4378 (based on amino acid composition)
STRUCTURE:5
The amino acid sequence and structure of human albumin have been determined. Human albumin is a protein with no carbohydrate content. It is a single polypeptide chain with one free sulfhydryl group on residue # 34 and 17 intrachain disulfide bonds.
Amino Acid Asp Asn Thr Ser Glu Gln Pro Gly Ala Cys Val Met Ile Leu Tyr Phe His Lys Trp Arg
Residues 39 15 30 22 60 23 25
PHYSICAL PROPERTIES:5 | 12 63 | 35 39 6 8 61 18 30 16 58 1 23 |
Sedimentation constant, S20,W X 1013 |
| 4.6 (monomer), 6.5 (dimer) |
Diffusion constant, D20,W X 107 |
| 6.1 |
Partial specific volume, V20 |
| 0.733 |
Intrinsic viscosity, ç |
| 0.042 |
Frictional ratio, f/f0 |
| 1.28 |
Overall dimensions, Å |
| 38 X 150 |
Isoelectric point (Ã/2 = 0.15) |
| 4.7 |
Isoionic point (Ã/2 = 0) |
| 5.2 |
Electrophoretic mobility, pH 8.6, Ã/2 = 0.15 |
| -5.9 |
Refractive index increment (578 nm) X 10-3 |
| 1.89 |
Optical absorbance, A279 nm (1 gram/liter) |
| 0.531 |
Mean residue rotation, [m']233 | 8590 |
|
Mean residue ellipticity |
| 17 [è]209 nm; 16 [è]222 nm |
Estimated á-helix, % |
| 48 |
Estimated â-form, % |
| 15 |
SOLUBILITY / SOLUTION STABILITY: |
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儲存與安全
儲存:
2-8℃
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