SBI無(wú)外泌體血清（原裝）小批量到貨

品牌：     SBI

貨號(hào)：     EXO-FBS-50A-1

供應(yīng)商：上海試劑銷(xiāo)售科技有限公司

數(shù)量：100

SBI無(wú)外泌體血清（原裝）小批量到貨保存條件：僅供科研使用，

-20度保存，使用時(shí)，解凍后4度保存無(wú)需加熱滅活。

規(guī)格：     50ml  250ml  500ml

    SBI無(wú)外泌體血清（原裝）Exosome是一種可由多種細(xì)胞分泌的直徑為30-150nm的膜性小囊泡。經(jīng)研究后發(fā)現(xiàn)，exosome其內(nèi)含有*的蛋白質(zhì)與核酸分子，exosome所含成份與其細(xì)胞來(lái)源密切相關(guān)，這也決定了exosome的功能特異性，如抗原提呈、信號(hào)傳導(dǎo)或誘導(dǎo)抗腫瘤免疫反應(yīng)、細(xì)胞遷移、細(xì)胞分化、細(xì)胞間通信、腫瘤轉(zhuǎn)移等。目前，exosome研究已經(jīng)成為臨床診斷與基礎(chǔ)醫(yī)生的新熱點(diǎn)。

    上海試劑銷(xiāo)售提供高質(zhì)量的、便捷的ExoPerfect系列exosome分離純化試劑盒，針對(duì)不同體液(血清、血漿、唾液、尿液、細(xì)胞培養(yǎng)上清等)，能高效獲取高純度的exosome，方便科研人員對(duì)exosome內(nèi)容物及生理功能進(jìn)行深入研究:

1.簡(jiǎn)單、便捷的ExoPerfectTM系列exosome分離純化試劑盒，針對(duì)不同體液(血清、血漿、唾液、尿液、細(xì)胞培養(yǎng)上清液等)，能高效獲取高純度的exosome，方便科研人員對(duì)exosome內(nèi)容物及生理功能進(jìn)行深入研究；

2.ExoPerfectTM系列exosome-freeFBS用于各種常規(guī)細(xì)胞培養(yǎng)，*避免FBS中的exosome對(duì)實(shí)驗(yàn)的干擾；

3.ExoPerfectTM系列exosome示蹤慢病毒，用于實(shí)時(shí)監(jiān)控exosome的行蹤，使exosome的去向一目了然；

4.特異性靶向exosome的慢病毒載體，可將客戶(hù)感興趣的目的基因靶向表達(dá)于exosome之上，方便客戶(hù)應(yīng)用exosome作為臨床疫苗及載藥系統(tǒng)的研究；

    SBI無(wú)外泌體血清（原裝）小批量到貨因產(chǎn)品特殊性，一次性從國(guó)外只能少量訂貨所以庫(kù)存有限，預(yù)購(gòu)從速！

細(xì)胞的分化、衰老與死亡

1.細(xì)胞的分化:一個(gè)成年人全身細(xì)胞總數(shù)約1012個(gè)，可以區(qū)分為200多種不同類(lèi)型的細(xì)胞：形態(tài)結(jié)構(gòu)，代謝，行為，功能等各不相同。追根溯源，這么多種細(xì)胞均來(lái)自一個(gè)受精卵細(xì)胞。所以，通常把發(fā)育過(guò)程中，細(xì)胞后代在形態(tài)、結(jié)構(gòu)和功能上發(fā)生差異的過(guò)程稱(chēng)為細(xì)胞分化。細(xì)胞分化發(fā)生在胚胎階段，也發(fā)生在胎兒出生以后，乃至成人階段。例如，人體血細(xì)胞的產(chǎn)生和分化，這個(gè)過(guò)程在人的一生中一直持續(xù)著。

    由旺盛生長(zhǎng)不斷分裂的細(xì)胞，轉(zhuǎn)入分化，通常從細(xì)胞周期中G1期開(kāi)始時(shí)一個(gè)確定的點(diǎn)G0點(diǎn)“逃逸”出細(xì)胞周期。旺盛生長(zhǎng)分裂的細(xì)胞和各種分化了的細(xì)胞，它們的基因表達(dá)和代謝活動(dòng)各不相同。

2.細(xì)胞的衰老:體外細(xì)胞培養(yǎng)實(shí)驗(yàn)證明：

    成纖維細(xì)胞：來(lái)自胎兒傳代50次后衰老死亡,來(lái)自成人傳代20次后衰老死亡(與具體年齡有關(guān));來(lái)自小鼠傳代14--28次后衰老死亡,來(lái)自烏龜傳代90--125次后衰老死亡。

    細(xì)胞衰老過(guò)程中會(huì)發(fā)生一系列的變化，包括：蛋白質(zhì)合成速度降低，已有蛋白質(zhì)結(jié)構(gòu)變化，特異蛋白質(zhì)成份出現(xiàn);同時(shí)，細(xì)胞核，線粒體，膜系統(tǒng)、骨架系統(tǒng)等都有結(jié)構(gòu)、功能的改變。

    細(xì)胞衰老原因，尚無(wú)定論，出現(xiàn)過(guò)好幾種理論與假說(shuō)，其中得到較廣泛認(rèn)可的是“自由基損傷假說(shuō)”。

Exo-FBS Overview: When You're Not Studying Bovine Exosomes   

Do you study bovine exosomes? If not, and you use fetal bovine serum (FBS) in your cell or tissue culture media, you may be isolating more bovine exosomes than you realize. Serum is a rich source of exosomes, FBS included. In order to help scientists study only the exosomes they want, SBI provides Exo-FBS™ exosome-depleted FBS.

· Exosome-sized vesicles removed

· Very low levels of CD63 positive cow exosome

· Undetectable levels of cow microRNAs

· Comparable growth rates as standard FBS

· Identical use as standard FBS (add 10% in DMEM or RPMI)

Background

Standard growth media for most cells in culture require FBS as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.

SBI has developed an exosome-depleted FBS growth supplement called Exo-FBS that has been stripped of bovine exosomes. Exo-FBS supports equivalent growth of many types of cells in culture, is devoid of cow CD63-positive exosomes, and does not have any measurable bovine microRNAs. Cell growth in Exo-FBS is comparable to standard FBS. The process of manufacturing of Exo-FBS is a patented method in Patent No.: US 9,005,888 B2.

Conclusions

Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments, and with no ultracentrifugation required when you use ExoQuick-TC. Available as standard FBS supplement or heat inactivated FBS media supplement (treated at 65°C for 15 minutes before bovine exosome removal).

SBI無(wú)外泌體血清|8月大促，所有生物試劑產(chǎn)品（胎牛血清、細(xì)胞、培養(yǎng)基、雙抗、胰酶、elisa試劑盒）超*.........