技術(shù)文章
家畜疾病系列elisa試劑盒被研制
閱讀:119 發(fā)布時間:2015-4-3獻血者抗-HCV酶聯(lián)免疫吸附反應(yīng),但負面的NAT和RIBA不一定是*遞延,他們可能會重新接納這些捐助者作出了巨大的貢獻,作為它答應(yīng)按期寶貴的念頭捐助者,其血液中已被證實是安全的。863計劃現(xiàn)代農(nóng)業(yè)專題在動植物無特定疫害關(guān)鍵技術(shù)創(chuàng)新上取得重要突破,完善了系列重要豬病診斷的elisa試劑盒,對進一步提高我國豬病的診斷水平將起到積極作用。豬繁殖與呼吸綜合征診斷與監(jiān)測elisa試劑盒、偽狂犬病gE鑒別elisa試劑盒、偽狂犬病gG鑒別elisa試劑盒診斷試劑盒的留存期試驗表明,的3批試劑盒均可以在2~8℃條件下留存6個月以上;豬瘟病毒E2和E0蛋白在真核表達系統(tǒng)中成功表達,桿狀病毒表達抗原的檢出率比原核抗原提高3-5倍,為豬瘟診斷與監(jiān)測elisa試劑盒的進一步大批提供了保障,在9個省市自治區(qū)推廣應(yīng)用120多個試劑盒,共檢測萬余份豬血清,豬瘟抗體陽性率在12%~56%之間,進一步證明了試劑盒具有可操作性強、穩(wěn)定性和重復(fù)性好的特點;實現(xiàn)了豬圓環(huán)病毒2型ORF2基因在巴斯德畢赤酵母中獲得了表達,進一步完善了豬日本乙型腦炎診斷elisa試劑盒、豬圓環(huán)病毒2型感染診斷與監(jiān)測elisa試劑盒和豬細小病毒病診斷與監(jiān)測ELISA的工藝和確定了試劑盒的反應(yīng)條件醫(yī)學(xué)教育`網(wǎng)搜集整理。收件人的血液制品從以前的抗-HCV ELISA呈陽性,基因- PCR呈陰性,RIBA不確定的或負的捐助者的捐募,沒有丙型肝炎病毒感染。這些捐助者沒有被感染,筆者曾建議,這些捐助者可以重新輸入,提供捐助,將來的捐贈抗-HCV ELISA呈陰性。
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