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92261ES60Recombinant Mouse Thrombopoietin, His (Mouse TPO,
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產(chǎn)品簡介
| 供貨周期 | 現(xiàn)貨 | 規(guī)格 | 100ug |
|---|---|---|---|
| 貨號 | 92261ES60 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè) |
產(chǎn)品介紹
Product Information
Product Name
Cat#
Size
Price（￥）
Recombinant Mouse Thrombopoietin, His (Mouse TPO, His)
92261ES60
100 μg
6875.00
92261ES80
1 mg
29895.00
Product Description
Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis. The 353 amino acid (aa) human TPO precursor is cleaved to yield the 332 aa mature protein. Mature human TPO shares approximately 70% aa sequence homology with mouse and rat TPO. TPO and its receptor (c-Mpl) are the major regulators of megakaryocyte and platelet production and serve a critical and non-redundant role in hematopoietic stem cell (HSC) biology. TPO signals through the Jak-STAT, Ras-Raf-MAPK, and PI3K pathways, and promotes survival, proliferation, and polyploidization in megakaryocytes. The proto-oncogene c- also plays an important role in many of these same processes.
Product Properties
Synonyms
MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, THCYT1, THPO
Accession
P40226
GeneID
21832
Source
HEK293-derived mouse TPO protein, Met1-Thr365.
Molecular Weight
Approximately 85.0 kDa.
AA Sequence
MELTDLLLA AMLLAVARL TLSSPVAPA CDPRLLNKL LRDSHLLHS RLSQCPDVD PLSIPVLLP AVDFSLGEW
KTQTEQSKA QDILGAVSL LLEGVMAAR GQLEPSCLS SLLGQLSGQ VRLLLGALQ GLLGTQLPL QGRTTAHKD
PNALFLSLQ QLLRGKVRF LLLVEGPTL CVRRTLPTT AVPSSTSQL LTLNKFPNR TSGLLETNF SVTARTAGP
GLLSRLQGF RVKITPGQL NQTSRSPVQ ISGYLNRTHG PVNGTHGLFA GTSLQTLE ASDISPGAFNKGSLAFNL
QGGLPPSPS LAPDGHTPF PPSPALPTT HGSPPQLHP LFPDPSTTM PNSTAPHPV TMYPHPRNL SQETTag
C-His
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 90% by SDS-PAGE analyses.
Biological Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220211
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