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您現(xiàn)在的位置: 翌圣生物科技(上海)股份有限公司>>蛋白與免疫>>蛋白酶>> 20433ESRecombinant Human Ubiquitin Activating Enzyme E1 (
20433ESRecombinant Human Ubiquitin Activating Enzyme E1 (
參考價1920
具體成交價以合同協(xié)議為準(zhǔn)
  • 20433ES 型號
  • Yeasen/翌圣生物 品牌
  • 生產(chǎn)商 廠商性質(zhì)
  • 上海市 所在地

規(guī)格
25 µg1920元10 件 可售

訪問次數(shù):189更新時間:2023-11-19 20:49:29

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初級會員·12年
聯(lián)人:
曹女士
話:
400-6111-883
機(jī):
后:
4006-111-883
真:
86-21-34615995
址:
上海市浦東新區(qū)天雄路166弄1號3樓
網(wǎng)址:
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產(chǎn)品簡介
供貨周期 現(xiàn)貨 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè),制藥
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating wi
產(chǎn)品介紹

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.

 

產(chǎn)品性質(zhì)

別名(Synonyms)

UBA1, UBA6

來源(Source)

E.coli

分子量

(Molecular Weight)

Approximately 118 kDa.

氨基酸序列

(AA Sequence)

MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT

SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY

DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP

GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

 

外觀
(Physical Appearance)

Liquid

標(biāo)簽(Tag)

None

純度(Purity)

> 95% by SDS-PAG

緩沖液(Buffer)

50mM PBS, pH7.0, 150mM NaCl, 1mM DTT.

 

儲存條件

-25~-15℃保存

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 本產(chǎn)品僅作科研用途

2. 為了您的安全和健康,請穿實(shí)驗(yàn)服并佩戴一次性手套操作。

反應(yīng)緩沖液配方

名稱

組分

濃度

 

10 × Reaction Buffer(200 ul)

Tris PH8.0

500 mM

DTT

6 mM

ddH2O

to 200μL

 

10 x ATP-Mg (200 ul)

 

ATP

100 mM

MgCl2

100 mM

ddH2O

to 200μL

 

參考陽性反應(yīng)體系

組分

體積 (μL)

ddH2O

to 20 μL

10 × Reaction Buffer

2 μL

10 × E1 Enzyme

2 μL

5 × Ubiquitin

4 μL

10 × ATP-Mg

2 μL

20 × E2 Enzyme

1 μL

10 × E3 Ligase Itch (Positive control)

2 μL

20 × S5a substrate (Positive control)

1 μL




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