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產品簡介
| 供貨周期 | 兩周 | 應用領域 | 醫(yī)療衛(wèi)生,生物產業(yè) |
|---|
產品介紹
產品信息
產品名稱 | 產品編號 | 規(guī)格 |
Recombinant Human R spondin 3/RSPO3 Protein,His Tag | 92283ES60 | 100 μg |
92283ES76 | 500 μg |
性能參數
表達區(qū)間及表達系統(tǒng)（Source） | Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1] |
分子量大?。?/span>Molecular Weight） | The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result. |
內毒素（Endotoxin） | Less than 1EU per μg by the LAL method. |
純度（Purity） | > 95% as determined by Tris-Bis PAGE |
活性（Activity） | ELISA Data: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA SPR Data: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) |
制劑（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重構方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
