公司總部位于匈牙利,雙鏈RNA分子單克隆抗體的杰出代理商??蛻羧喊ㄖ难芯克诿绹?0個(gè)州,超過一半分布有SCICONS的客戶,客戶群遍布世界各地。
雙鏈RNA抗體廣泛應(yīng)用于檢測(cè)病毒dsRNA 中間體,例如:丙型肝炎病毒、登革熱病毒、小鼠腦脊髓炎病毒、鼻病毒、基孔肯雅病毒、狂犬病病毒、脊髓灰質(zhì)炎病毒、豬瘟病毒、雀麥草花葉病毒檢測(cè)樣本可以是細(xì)胞培養(yǎng)物或石蠟包埋的組織。
應(yīng)用SCICONS公司抗雙鏈RNA抗體,已發(fā)表文章300余篇。
J2抗雙鏈RNA IgG2a單抗是雙鏈RNA檢測(cè)的金標(biāo)準(zhǔn)。起初只用于植物病毒檢測(cè)研究,但從2006年Weber et al,發(fā)表文章表明:在檢測(cè)的感染細(xì)胞內(nèi)所有的正鏈RNA病毒均產(chǎn)生大量雙鏈RNA,隨后該抗體被應(yīng)用于廣泛的檢測(cè)系統(tǒng),發(fā)表文章超過200余篇。
J2抗體用于檢測(cè)病毒dsRNA 中間體,例如:丙型肝炎病毒、登革熱病毒、小鼠腦脊髓炎病毒、鼻病毒、基孔肯雅病毒、狂犬病病毒、脊髓灰質(zhì)炎病毒、豬瘟病毒、雀麥草花葉病毒檢測(cè)樣本可以是細(xì)胞培養(yǎng)物或石蠟包埋的組織。
J2抗體被廣泛應(yīng)用于電鏡學(xué)、免疫熒光顯微技術(shù)、免疫組化及各種免疫捕捉實(shí)驗(yàn),如:斑點(diǎn)免疫印跡、ELISA。J2抗體是用于區(qū)分被檢樣本是病毒或細(xì)菌的有效工具((Richardson et al., 2010))。近,J2用于制備mRNA過程中dsRNA的監(jiān)測(cè),因此該抗體基因治療中,有潛在的應(yīng)用價(jià)值。
應(yīng)用實(shí)例:
參考文獻(xiàn):
Fang, C. Y., Chen, S. J., Wu, H. N., Ping, Y. H., Lin, C. Y., Shiuan, D., Chen, C. L., Lee, Y. R., Huang, K. J. (2015) Honokiol, a Lignan Biphenol Derived from the Magnolia Tree, Inhibits Dengue Virus Type 2 Infection Viruses 7: 4894-910
Cheng, X., Deng, P., Cui, H., Wang, A. (2015) Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation Virology 485: 439-451
Kreit, M., Vertommen, D., Gillet, L., Michiels, T. (2015) The Interferon-Inducible Mouse Apolipoprotein L9 and Prohibitins Cooperate to Restrict Theiler's Virus Replication PLoS One 10: e0133190
Xie, W., Wang, L., Dai, Q., Yu, H., He, X., Xiong, J., Sheng, H., Zhang, D., Xin, R., Qi, Y., Hu, F., Guo, S., Zhang, K. (2015) Activation of AMPK restricts coxsackievirus B3 replication by inhibiting lipid accumulation J Mol Cell Cardiol 85: 155-167
Wan, J., Cabanillas, D. G., Zheng, H., Laliberté, J. F. (2015) Turnip mosaic virus moves systemically through both phloem and xylem as membrane-associated complexes Plant Physiol 167: 1374-88
K1單克隆抗體,IgG2a亞型,能夠識(shí)別dsRNA(雙鏈RNA)。K1抗體可用于檢測(cè)細(xì)胞和組織中的dsRNA.經(jīng)證明,K1抗體是J2很好的替代品。在一些特殊的實(shí)驗(yàn)中,用K1代替J2抗體,可以明顯解決交叉反應(yīng)或者是高背景。
K1抗體可以檢測(cè)病毒dsRNA中間體,例如肝炎病毒、小鼠腦脊髓病毒、乙型腦炎病毒等。K1抗體還可以檢測(cè)培養(yǎng)細(xì)胞以及石蠟包埋組織中的dsRNA。
如果要檢測(cè)Poly I:C(多聚肌苷酸),我們強(qiáng)烈推薦使用K1抗體而非J2抗體,因?yàn)?/span>K1與合成的多核糖核苷酸有很強(qiáng)的親和力。
K1抗體被廣泛應(yīng)用于免疫熒光、流式細(xì)胞術(shù)(FACS)和免疫捕捉檢測(cè),例如斑點(diǎn)免疫印跡及ELISA實(shí)驗(yàn)。
應(yīng)用實(shí)例:
參考文獻(xiàn):
Takamatsu, Y., Uchida, L., Morita, K. (2015) Delayed interferon response differentiates replication of West Nile virus and Japanese encephalitis virus in human neuroblastoma and glioblastoma cells J Gen Virol
Kreit, M., Vertommen, D., Gillet, L., Michiels, T. (2015) The Interferon-Inducible Mouse Apolipoprotein L9 and Prohibitins Cooperate to Restrict Theiler's Virus Replication PLoS One 10: e0133190
Hu, Z., Leppla, S. H., Li, B., Elkins, C. A. (2014) Antibodies specific for nucleic acids and applications in genomic detection and clinical diagnostics Expert Rev Mol Diagn : 1-22
Kawashima, T., Kosaka, A., Yan, H., Guo, Z., Uchiyama, R., Fukui, R., Kaneko, D., Kumagai, Y., You, D. J., Carreras, J., Uematsu, S., Jang, M. H., Takeuchi, O., Kaisho, T., Akira, S., Miyake, K., Tsutsui, H., Saito, T., Nishimura, I., Tsuji, N. M. (2013) Double-stranded RNA of intestinal commensal but not pathogenic bacteria triggers production of protective interferon-beta Immunity 38: 1187-97
Bernasconi, R., Galli, C., Noack, J., Bianchi, S., de Haan, C. A., Reggiori, F., Molinari, M. (2012) Role of the SEL1L:LC3-I complex as an ERAD tuning receptor in the mammalian ER Mol Cell 46: 809-19
K2單克隆抗體,lgM亞型,是抗dsRNA的單克隆抗體。
K2抗體主要用于一些特殊的檢測(cè)。K2抗體主要用于ELISA和夾心法ELISA實(shí)驗(yàn),一般來說,K2抗體只能用于檢測(cè)IgG(IgG2a)亞型抗體不適合的雙鏈RNA(dsRNA)。
K2抗體需干冰運(yùn)輸,因?yàn)楦邼舛鹊?/span>K2抗體容易發(fā)生凝聚,因?yàn)椴蝗菀鬃龀蓛龈煞郏荒艹剡\(yùn)輸。(J2和K1可常溫運(yùn)輸。)
參考文獻(xiàn)
Hu, Z., Leppla, S. H., Li, B., Elkins, C. A. (2014) Antibodies specific for nucleic acids and applications in genomic detection and clinical diagnostics Expert Rev Mol Diagn : 1-22
Szego, A., Enunlu, N., Deshmukh, S. D., Veliceasa, D., Hunyadi-Gulyas, E., Kuhne, T., Ilyes, P., Potyondi, L., Medzihradszky, K., Lukacs, N. (2010) The genome of Beet cryptic virus 1 shows high homology to certain cryptoviruses present in phylogenetically distant hosts Virus Genes 40: 267-76
Bokarewa, M., Tarkowski, A., Lind, M., Dahlberg, L., Magnusson, M. (2008) Arthritogenic dsRNA is present in synovial fluid from rheumatoid arthritis patients with an erosive disease course Eur J Immunol 38: 3237-44
Fontana, J., Tzeng, W. P., Calderita, G., Fraile-Ramos, A., Frey, T. K., Risco, C. (2007) Novel replication complex architecture in rubella replicon-transfected cells Cell Microbiol 9: 875-90
McCartney, A. W., Greenwood, J. S., Fabian, M. R., White, K. A., Mullen, R. T. (2005) Localization of the tomato bushy stunt virus replication protein p33 reveals a peroxisome-to-endoplasmic reticulum sorting pathway Plant Cell 17: 3513-31
產(chǎn)品訂購:
貨號(hào) | 抗體名稱 | 規(guī)格 |
10010200 | J2單抗 | 200 μg |
10010500 | 500 μg | |
10020200 | K1單抗 | 200 μg |
10020500 | 500 μg | |
10030005 | K2單抗 | 5ml |
10030010 | 10 ml | |
10040200 | J5單抗 | 200 μg |
10040500 | 500 μg | |
10050100 | anti-dsRNA mAb Comparison Kit | 3 x 100 µg |
10613002 | dsRNA Detection Kit (J2 based) | 2 plates |
10623002 | dsRNA Detection Kit (K1 based) | 2 plates |
10613005 | dsRNA Detection Kit (J2 based) | 5 plates |
10623005 | dsRNA Detection Kit (K1 based) | 5 plates |
10080100 | dsRNA (142 bp, Positive Control) | 50 µg |
溫馨提示:
1)J2、K1單克隆抗體由于凍干過程中,稀釋的抗體可能含有少量的變性蛋白聚合物,這些蛋白可能會(huì)對(duì)免疫組化實(shí)驗(yàn)產(chǎn)生高背景。因此,我們推薦在使用前,抗體重懸后,需離心,使用其上清。
2)J2、K1可常溫運(yùn)輸,稀釋后請(qǐng)分裝,-20 ℃或-70℃保存,盡量避免反復(fù)凍融。
3)K2需干冰運(yùn)輸,到貨后請(qǐng)分裝,-70℃保存。
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