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當(dāng)前位置:上海信裕生物科技有限公司>>抗體>>一抗>> Insulin (INS) 抗體
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更新時(shí)間:2018-01-29 01:27:23瀏覽次數(shù):456次
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抗原
Insulin (INS)
別名 ins1, xins, ins1-a, Insulin, IDDM2, ILPR, IRDN, MODY10, AA986540, Ins-2, InsII, Mody, Mody4, proinsulin, zgc:109842
適用
其他選擇Human, Pig (Porcine)
宿主
其他選擇Mouse
克隆類型 (克隆位點(diǎn))
單克隆 (2D11-H5)
標(biāo)記
其他選擇非結(jié)合性
應(yīng)用范圍
其他選擇Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
[Pubmed] 1 reference available
規(guī)格 0.1 mg
發(fā)貨至 中國(guó) (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日
Insulin (INS) 抗體
產(chǎn)品細(xì)節(jié)
引用圖像貯存及處理使用細(xì)節(jié)目標(biāo)詳細(xì)情況回頁(yè)首
免疫原 Purified human Insulin coupled to Bovine Serum Albumin (BSA).
序列 Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN
克隆位點(diǎn) 2D11-H5
亞型 IgG1
特異性 This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:
純化方法 Protein A Chromatography
Insulin (INS) 抗體目標(biāo)詳細(xì)情況
引用圖像貯存及處理使用細(xì)節(jié)回頁(yè)首
別名 Insulin
背景 Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS
分子量 6 kDa
基因ID 3630
NCBI Accession NP_000198
UniProt P01308
研究領(lǐng)域 Neurology, Diabetes, Hormones
使用細(xì)節(jié)
引用圖像貯存及處理目標(biāo)詳細(xì)情況回頁(yè)首
應(yīng)用備注 This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60?minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10?μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imay 5?ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).
限制 For Research Use only
Insulin (INS) 抗體貯存及處理
引用圖像使用細(xì)節(jié)目標(biāo)詳細(xì)情況回頁(yè)首
濃度 1.0 mg/mL (by UV absorbance at 280 nm)
緩沖液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride,?pH 7.2, 0.01?% (w/v) Sodium Azide, None
儲(chǔ)存液 Sodium azide
注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項(xiàng) Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
儲(chǔ)存條件 -20 °C
儲(chǔ)存方法 Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
xy-3050R Phospho-Aurora A (Thr288)磷酸化有絲分裂激酶A抗體
xy-3003R phospho-APS(Ser598)磷酸化APS抗體
xy-2749R Aurora A有絲分裂激酶A抗體
xy-5986R AIM2干擾素誘導(dǎo)蛋白AIM2抗體
xy-4611R Aquaporine 2水通道蛋白-2抗體
xy-5984R ABI2腫瘤抑制基因ABI2/精氨酸酪氨酸蛋白激酶結(jié)合蛋白抗體
xy-5987R ARL11ADP核糖基化因子樣11抗體
xy-5990R ARMCX3ARM重復(fù)X連鎖蛋白ARMCX3抗體
xy-5988R ARMCX1ARM重復(fù)X連鎖蛋白ARMCX1抗體
xy-5989R ARMCX2ARM重復(fù)X連鎖蛋白ARMCX2抗體
xy-5991R Apg10自噬相關(guān)蛋白10抗體
xy-6017R ACBD6?;o酶A結(jié)合結(jié)構(gòu)域蛋白6抗體
xy-6019R Aminoacylase 1氨基?;?抗體
xy-3831R Adipose Triglyceride Lipase脂肪甘油三酯脂酶抗體
xy-4002R phospho-AMPK alpha-1 (Thr172)磷酸化腺苷單磷酸活化蛋白激酶α1抗體
xy-3847R Amphiregulin雙調(diào)蛋白(結(jié)腸直腸細(xì)胞源性生長(zhǎng)因子)抗體
xy-5185R phospho-AQP2(Ser264)磷酸化水通道蛋白2抗體
xy-5180R AXL粘附相關(guān)激酶抗體
xy-5181R phospho-AXL (Tyr698+Tyr702+Tyr703)磷酸化粘附相關(guān)激酶抗體
xy-5182R phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305)磷酸化蛋白激酶B抗體
xy-5187R phospho-AQP2(Ser269)磷酸化水通道蛋白2抗體
xy-5186R phospho-Amyloid Precursor Protein (Thr743)磷酸化APP淀粉樣肽前體蛋白抗體
xy-5188R phospho-AKT1(Ser129)磷酸化蛋白激酶B抗體
xy-5189R phospho-Amyloid Precursor Protein (Ser198)磷酸化APP淀粉樣肽前體蛋白抗體
xy-5190R phospho-ATF4 (Ser245)磷酸化活化轉(zhuǎn)錄因子4抗體
xy-5191R phospho-Androgen Receptor (Ser213)磷酸化雄激素受體抗體
xy-5192R phospho-Androgen Receptor (Ser650)磷酸化雄激素受體抗體
xy-5193R phospho-AKT1+2+3 (Tyr315+316+312) 磷酸化蛋白激酶AKT1,2,3抗體
xy-5194R phospho-AKT1(Thr34)磷酸化蛋白激酶B抗體
xy-5195R phospho-ATF2(Ser472)磷酸化活化復(fù)制因子2抗體
xy-9806R APR3凋亡相關(guān)蛋白3抗體
xy-3871R ATG18自噬相關(guān)蛋白18抗體
xy-3873R ATG4A自噬相關(guān)蛋白4A抗體
xy-3876R APOA4載脂蛋白A4抗體
xy-3874R ALOX1212脂氧合酶抗體
xy-3877R Angiotensin III血管緊張素III抗體
xy-4021R ADCY3腺苷酸環(huán)化酶3抗體
xy-4171R Angiomotin血管動(dòng)蛋白抗體
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