FGF7抗體

抗原
Fibroblast Growth Factor 7 (FGF7)
別名 KGF, HBGF-7, kgf, Kgf, FGF-7
適用
其他選擇 Human
Human(58),Murine (16),Rat ((Rattus)) (15),Chicken (13),Dog (Canine) (13),Pig (Porcine) (13),Sheep (Ovine) (1)
宿主
其他選擇 Rabbit
Rabbit(46),Mouse (11),Goat (1)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(6),FITC (2),HRP (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),Magnetic Particles (1),PE (1),Sepharose (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(45),ELISA (23),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (IHC) (9),Neutralization (Neut) (5),Enzyme Immunoassay (EIA) (4),Immunoprecipitation (IP) (4),Functional Studies (Func) (3),Blocking Antibody (Inhibition) (2),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (2),Immunocytochemistry (ICC) (1),Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
[Pubmed] 1 reference available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

FGF7抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Highly purified (>98%) recombinant FGF-7 (KGF)
特異性 This antibody reacts with Human Keratinocyte Growth Factor (KGF).
純化方法 Affinity chromatography

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 FGF7 / KGF
背景 KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor
基因ID 2252
NCBI Accession NP_002000
UniProt P21781

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25?ng/mL), a concentration of 0.56-0.85?μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4?ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2?μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0?ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

顯示 隱藏
溶解方式 Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
緩沖液 PBS,?pH 7.2 without preservatives
儲存液 Without preservative
注意事項(xiàng) Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
儲存條件 4 °C/-20 °C
儲存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

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