RAD17 抗體

抗原
RAD17 Homolog (S. Pombe) (RAD17)
別名 zgc:91969, RAD17, K2A18.21, K2A18_21, RADIATION SENSITIVE 17, CCYC, HRAD17, R24L, RAD17SP, RAD24, 9430035O09Rik, MmRad24
抗原表位
其他選擇 AA 218-246, Center
pSer645(24),AA 1-110 (8),AA 225-254 (8),C-Term (6),Internal Region (6),AA 2-15 (5),N-Term (5),AA 1-584 (3),AA 10-23 (3),Ser645 (3),pSer646 (2),pSer656 (2),AA 1-200 (1),AA 151-423 (1),AA 2-240 (1),AA 218-246 (1),AA 389-681 (1),AA 592-681 (1),AA 621-670 (1),AA 644-652 (1),Ala639 (1),Middle Region (1),Thr526 (1),Thr534 (1),pSer647 (1)
適用
其他選擇 Human
Human(117),Murine (56),Rat ((Rattus)) (39),Bovine (14),Dog (Canine) (14),Pig (Porcine) (12),Schizosaccharomyces pombe (3),Yeast (3)
宿主
其他選擇 Rabbit
Rabbit(108),Mouse (15),Goat (5),Sheep (1)
克隆類型 (克隆位點(diǎn))
多克隆 (RB20035)
標(biāo)記
其他選擇 非結(jié)合性
Biotin(4),FITC (4),HRP (4),APC (2),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Alkaline Phosphatase (AP) (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),PE (2)
應(yīng)用范圍
其他選擇 Western Blotting (WB), Flow Cytometry (FACS)
Western Blotting (WB)(111),ELISA (45),Flow Cytometry (FACS) (20),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunofluorescence (IF) (17),Immunohistochemistry (IHC) (12),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12),Immunocytochemistry (ICC) (5),Immunoprecipitation (IP) (4),Immunoassay (IA) (3),Enzyme Immunoassay (EIA) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 2 references available
規(guī)格 400 μL
發(fā)貨至 中國 (更改)
發(fā)貨時間 9至13個工作日

RAD17 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 AA from the Central region of human RAD17.
克隆位點(diǎn) RB20035
亞型 Ig
特異性 This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of human RAD17.
純化方法 This antibody is purified through a protein A column, followed by peptide affinity purification.

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 RAD17
背景 The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Synonyms: RB20035
分子量 77055 DA
基因ID 5884
NCBI Accession NP_579916, NP_579917, NP_579918, NP_579919, NP_002864, NP_579920, NP_579922
UniProt O75943
研究領(lǐng)域 Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Cell Structure

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使用細(xì)節(jié)

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應(yīng)用備注 WB = 1:1000, FACS = 1:10-50
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
濃度 0.46 mg/mL
緩沖液 PBS with 0.09 % (W/V) sodium azide
儲存液 Sodium azide
注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
儲存條件 4 °C/-20 °C
儲存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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