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更新時(shí)間:2018-01-27 19:59:46瀏覽次數(shù):332次
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抗原
RAD54 Homolog B (S. Cerevisiae) (RAD54B)
別名 RAD54B, im:7137737, im:7153525, fsbp, rdh54, RDH54, E130016E03Rik, Fsbp, RGD1306507
抗原表位
其他選擇 N-Term
AA 801-910(8),N-Term (4),AA 1-158 (3),Internal Region (2),AA 241-290 (1),AA 580-910 (1),C-Term (1),Middle Region (1)
適用
其他選擇 Human
Human(42),Murine (15),Rat ((Rattus)) (14),Chicken (13),Bovine (12),Dog (Canine) (12),Xenopus laevis (1)
宿主
其他選擇 Rabbit
Rabbit(33),Mouse (9)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(2),FITC (2),HRP (2),APC (1),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),PE (1)
應(yīng)用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(26),ELISA (14),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunocytochemistry (ICC) (7),Immunohistochemistry (IHC) (7),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5),Immunoassay (IA) (3),Immunofluorescence (IF) (3),Immunoprecipitation (IP) (2)
[Pubmed] 1 reference available
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 9至12個(gè)工作日
RAD54B 抗體
產(chǎn)品細(xì)節(jié)
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產(chǎn)品細(xì)節(jié)
顯示 隱藏
免疫原 Synthetic peptide directed towards the N terminal of human RAD54B
序列 RRSAAPSQLQ GNSFKKPKFI PPGRSNPGLN EEITKLNPDI KLFEGVAINN
預(yù)測(cè)反應(yīng) Human : 100 %, Bovine : 90 %
產(chǎn)品特性 This is a rabbit polyclonal antibody against RAD54B. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified
目標(biāo)詳細(xì)情況
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目標(biāo)詳細(xì)情況
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別名 RAD54B
背景 RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.
分子量 103 kDa
基因ID 25788
NCBI Accession NP_036547, NM_012415
UniProt Q9Y620
研究領(lǐng)域 Cancer, Cell Cycle, DNA/RNA
使用細(xì)節(jié)
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使用細(xì)節(jié)
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應(yīng)用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明
Antigen size: 910 AA
限制 For Research Use only
貯存及處理
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貯存及處理
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狀態(tài) Lyophilized
溶解方式 Add 50 µL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項(xiàng) Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 -20 °C
儲(chǔ)存方法 For longer periods of storage, store at -20 °C
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