CRISP1抗體

抗原
Cysteine-Rich Secretory Protein 1 (CRISP1)
別名 9230112K08Rik, crisp-1, AEGL1, ARP, CRISP-1, HSCRISP1D, HSCRISP1G, HUMARP, AW324342, Aeg1, Aeg, Crisp-1, AEG
抗原表位
其他選擇 N-Term
C-Term(9),AA 161-191 (7),N-Term (5),AA 162-191 (2),AA 1-249 (1),AA 20-244 (1)
適用
其他選擇 Pig (Porcine), Bovine, Human
Human(17),Murine (1)
宿主
其他選擇 Rabbit
Rabbit(15),Mouse (2),Sheep (1)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
APC(1),Alkaline Phosphatase (AP) (1),Biotin (1),FITC (1),HRP (1),PE (1)
應用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(17),ELISA (6),Immunohistochemistry (IHC) (2),Immunoassay (IA) (1)
[Pubmed] 1 reference available
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時間 9至12個工作日

CRISP1抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

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免疫原 Synthetic peptide directed towards the N terminal of human CRISP1
序列 LKMSWSEEAA QNARIFSKYC DMTESNPLER RLPNTFCGEN MHMTSYPVSW
預測反應 Bovine : 84 %, Goat : 84 %, Pig : 84 %, Rabbit : 74 %
產(chǎn)品特性 This is a rabbit polyclonal antibody against CRISP1. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified

目標詳細情況

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目標詳細情況

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別名 CRISP1
背景 Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. CRISP1 is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface.Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.
分子量 27 kDa
基因ID 167
NCBI Accession NP_001122, NM_001131
UniProt P54107
研究領域 Reproduction

使用細節(jié)

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使用細節(jié)

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應用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明

Antigen size: 249 AA

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Lyophilized
溶解方式 Add 50 µL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項 Avoid repeated freeze-thaw cycles.
儲存條件 -20 °C
儲存方法 For longer periods of storage, store at -20 °C

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