GLE1抗體 

抗原
GLE1 RNA Export Mediator Homolog (Yeast) (GLE1)
別名 DmelCG14749, GLE1, GLE1L, LCCS, LCCS1, hGLE1, Gle1l, 4933405K21Rik, AA553313
抗原表位
其他選擇 N-Term
C-Term(9),Middle Region (4),Center (3),AA 414-646 (2),Internal Region (2),AA 1-698 (1),AA 116-411 (1),AA 140-240 (1),AA 414-656 (1),N-Term (1)
適用
其他選擇 Murine, Rat ((Rattus)), Dog (Canine), Bovine, Human, Chicken
Human(41),Murine (28),Rat ((Rattus)) (28),Bovine (12),Dog (Canine) (12),Pig (Porcine) (12)
宿主
其他選擇 Rabbit
Rabbit(38),Mouse (3)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Alexa Fluor 350(1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Biotin (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),FITC (1),HRP (1)
應(yīng)用范圍
其他選擇 Western Blotting (WB)
Western Blotting (WB)(32),ELISA (18),Immunohistochemistry (IHC) (13),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Enzyme Immunoassay (EIA) (4),Immunofluorescence (IF) (4),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (4),Immunocytochemistry (ICC) (2),Immunoassay (IA) (1)
[Pubmed] 1 reference available
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時間 9至12個工作日

GLE1抗體 

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

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免疫原 Synthetic peptide directed towards the N terminal of human GLE1
序列 LKLREAER VKQAEQERLR KEEGQIRLRA LYALQEEMLQ LSLDASEQ
預(yù)測反應(yīng) Horse : 100 %, Human : 100 %, Bovine : 90 %, Dog : 90 %, Mouse : 90 %, Rat : 90 %, Chicken : 80 %
產(chǎn)品特性 This is a rabbit polyclonal antibody against GLE1. It was validated on Western Blot using a cell lysate as a positive control.
純化方法 Affinity Purified

 目標(biāo)詳細情況

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目標(biāo)詳細情況

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別名 GLE1
背景 GLE1 is a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
分子量 75 kDa
基因ID 2733
NCBI Accession NM_001499, NP_001490
UniProt Q53GS7
研究領(lǐng)域 Chromatin and Nuclear Signaling, DNA/RNA

 使用細節(jié)

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使用細節(jié)

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應(yīng)用備注 Optimal working dilutions should be determined experimentally by the investigator.
說明

Antigen size: 659 AA

限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Lyophilized
溶解方式 Add 50 µL of distilled water.
濃度 1 mg/mL
緩沖液 PBS buffer with 2 % sucrose
注意事項 Avoid repeated freeze-thaw cycles.
儲存條件 -20 °C
儲存方法 For longer periods of storage, store at -20 °C

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