Phosphotyrosine 抗體

抗原
Phosphotyrosine
適用
其他選擇 Human
Chemical(159),Human (49),Rat ((Rattus)) (32),Murine (28),Dog (Canine) (16),All Species (10),Armenian Hamster (8),Rabbit (8),Broad Bean (Vicia faba) (3),Amino Acid (1),Chicken (1),Bovine (1)
宿主
其他選擇 Mouse
Mouse(177),Rabbit (51),Goat (2)
克隆類型 (克隆位點)
單克隆 (SPM102)
標(biāo)記
其他選擇 非結(jié)合性
Biotin(27),HRP (21),FITC (17),PE (10),Alkaline Phosphatase (AP) (6),Agarose Beads (5),Rhodamine (2),TAMRA (2),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 633 (1),DyLight 650 (1),DyLight 680 (1),DyLight 755 (1),PerCP (1)
應(yīng)用范圍
其他選擇 Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(167),Immunoprecipitation (IP) (88),ELISA (73),Immunohistochemistry (IHC) (37),Enzyme Immunoassay (EIA) (34),Immunofluorescence (IF) (33),Flow Cytometry (FACS) (27),Immunocytochemistry (ICC) (22),Immunohistochemistry (Frozen Sections) (IHC (fro)) (15),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Dot Blot (DB) (3),Radioimmunoassay (RIA) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Purification (Purif) (1)
規(guī)格 0.5 mL
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Phosphotyrosine 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Phosphotyrosine conjugated to protein carriers.
克隆位點 SPM102
亞型 IgG1
產(chǎn)品特性 Cellular Localization: Membrane, cytoplasm
純化方法 Purified by Protein A/G

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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背景 Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. This antibody shows no cross-reaction with other phosphor-amino acids and is excellent for multiple applications including staining of formalin/paraffin tissues.
分子量 Varies
研究領(lǐng)域 Signaling, Metabolism, Amino Acids, Protein Modifications

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 IHC Dilution: 1:20
說明

Positive Control:
IHC (p): MCF-7 cells, EGF-stimulated A431 cells, breast carcinoma

實驗流程 IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:20. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Note: Milk-derived blocking solutions reportedly contain phosphoproteins that may inhibit phosphoamino acid antibody binding and therefore should be avoided.
限制 For Research Use only

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貯存及處理

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狀態(tài) Liquid
緩沖液 mouse monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
儲存液 Sodium azide
注意事項 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事項 Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
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