LAYN抗體

抗原
Layilin (LAYN)
別名 LAYN, si:dkey-27p23.2, E030012M19Rik, Gm511, RGD1564444
抗原表位
其他選擇 AA 1-220
AA 1-220(7),N-Term (4),AA 22-220 (2),AA 1-374 (1),AA 22-235 (1),Internal Region (1),Middle Region (1)
適用
其他選擇 Human
Human(50),Murine (30),Rat ((Rattus)) (28),Bovine (25),Dog (Canine) (24),Chicken (12),Pig (Porcine) (12)
宿主
其他選擇 Rabbit
Rabbit(42),Mouse (10)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Alexa Fluor 350(2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Biotin (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),FITC (2),HRP (2)
zui低檢測濃度 0.00245 ng/well
應(yīng)用范圍
其他選擇 ELISA
Western Blotting (WB)(25),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),ELISA (13),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8),Flow Cytometry (FACS) (3),Immunofluorescence (IF) (3),Immunoassay (IA) (1)
[Pubmed] 3 references available
規(guī)格 100 μg
發(fā)貨至 中國 (更改)

LAYN抗體

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產(chǎn)品細(xì)節(jié)

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亞型 IgG
特異性 Human Layilin / LAYN
產(chǎn)品特性 Produced in rabbits immunized with purified, recombinant Human Layilin / LAYN extracellular domain (rh Layilin, NP_849156.1, Met 1-Glu 220). Total IgG was purified by Protein A affinity chromatography .
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 LAYN
背景 Layilin, a recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, a ten-amino acid motif in the layilin cytoplasmic domain is sufficient for talin binding, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Furthermore, talin binds layilin, PIPK1gamma and integrins in similar although subtly different ways. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that layilin may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. In addition, layilin might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.
NCBI Accession NP_849156.1
研究領(lǐng)域 Signaling, Extracellular Matrix

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA ELISA: 0.5-1.0 μg/mL This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human Layilin. The detection limit for Human Layilin is 0.00245 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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