ICAM1抗體

抗原
Intercellular Adhesion Molecule 1 (ICAM1)
別名 BB2, CD54, P3.58, Icam-1, Ly-47, MALA-2, ICAM, ICAM-1, ICAM1
抗原表位
其他選擇 AA 1-480
pTyr512(38),AA 1-480 (15),C-Term (13),AA 1-485 (10),AA 1-493 (10),AA 28-480 (7),N-Term (6),AA 1-532 (5),Tyr512 (4),AA 301-532 (3),AA 510-514 (3),Middle Region (3),AA 253-267 (2),AA 28-483 (2),AA 41-464 (2),AA 46-301 (2),AA 1-371 (1),AA 1-50 (1),AA 1-533 (1),AA 198-537 (1),AA 214-532 (1),AA 26-480 (1),AA 328-465 (1),AA 339-523 (1),AA 457-506 (1),AA 467-517 (1),AA 479-528 (1),AA 488-514 (1),Domain D1 (1),Gln506 (1),Internal Region (1),Ser518 (1)
適用
其他選擇 Human
Human(466),Murine (121),Rat ((Rattus)) (69),Bovine (8),Dog (Canine) (2),Monkey (1)
宿主
其他選擇 Mouse
Mouse(305),Rabbit (215),Rat (68),Sheep (6),Hamster (4),Goat (3)
克隆類型 (克隆位點(diǎn))
單克隆 (1)
標(biāo)記
其他選擇 非結(jié)合性
Biotin(56),FITC (53),PE (50),APC (9),Alexa Fluor 488 (7),Alexa Fluor 647 (7),HRP (6),Alexa Fluor 350 (5),Alexa Fluor 555 (5),Cy3 (5),Cy5 (5),Cy5.5 (5),Cy7 (5),PerCP (3),Alexa Fluor 546 (2),DyLight 488 (1),DyLight 550 (1),DyLight 594 (1),DyLight 650 (1),DyLight 755 (1),Magnetic Particles (1),Sepharose (1)
應(yīng)用范圍
其他選擇 ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Flow Cytometry (FACS)(331),Western Blotting (WB) (231),ELISA (135),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (107),Immunoprecipitation (IP) (99),Immunohistochemistry (Frozen Sections) (IHC (fro)) (96),Immunohistochemistry (IHC) (64),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (50),Immunofluorescence (IF) (37),Immunocytochemistry (ICC) (35),Functional Studies (Func) (29),Blocking Reagent (BR) (16),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (16),Enzyme Immunoassay (EIA) (12),Immunoassay (IA) (6),Immunohistochemistry (Fixed) (IHC (fx)) (5),Dot Blot (DB) (4),Blocking Antibody (Inhibition) (3),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3),ELISA (Capture) (2),ELISA (Detection) (2),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (1),Intracellular Staining (ICS) (1),Proximity Ligation Assay (PLA) (1)
規(guī)格 50 μg
發(fā)貨至 中國 (更改)

ICAM1抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Recombinant Human ICAM1 / CD54 protein
克隆位點(diǎn) 1
亞型 IgG1
特異性 Human ICAM1 / CD54
交叉反應(yīng) （詳細(xì)） No cross-reactivity in ELISA with Human ICAM2 Human ICAM3 Human VCAM1 Human CD62E Human CEACAM1 Human cell lysate (293 cell line)
產(chǎn)品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54, Met 1-Glu 480, NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 ICAM1
背景 ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constituy present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration.ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum- infected erythrocytes (PFIE), providing more of a role in infectious disease.
NCBI Accession NP_000192.2

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使用細(xì)節(jié)

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應(yīng)用備注 Flow Cytometry - Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-α (50 ng/ml, 18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with 1 μl purified human ICAM1 (10346-MM01, 1 μg/test), then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.
限制 For Research Use only

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲(chǔ)存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 4 °C/-20 °C/-80 °C
儲(chǔ)存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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