FCGR3B 抗體

抗原
Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B)
別名 CD16, CD16b, FCG3, FCGR3, FCR-10, FCRIII, FCRIIIb
抗原表位
其他選擇 AA 1-200
AA 1-200(7),FG loop (3),AA 12-47 (2),N-Term (2),AA 20-208 (1)
適用
其他選擇 Human
Human(30),Pig (Porcine) (4)
宿主
其他選擇 Mouse
Mouse(24),Rabbit (9),Rat (1)
克隆類型 (克隆位點(diǎn))
單克隆 (7)
標(biāo)記
其他選擇 非結(jié)合性
FITC(8),PE (2),Biotin (1)
應(yīng)用范圍
其他選擇 Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS)(22),Western Blotting (WB) (8),ELISA (7),Immunoprecipitation (IP) (4),Functional Studies (Func) (1),Immunofluorescence (IF) (1),Immunohistochemistry (Acetone-fixed) (IHC (af)) (1),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
規(guī)格 50 μg
發(fā)貨至 中國 (更改)

FCGR3B 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Recombinant Human CD16b / FCGR3B protein
克隆位點(diǎn) 7
亞型 IgG2a
特異性 Human CD16b / FCGR3B
交叉反應(yīng) （詳細(xì)） Has cross-reactivity in ELISA with Human CD16a No cross-reactivity in ELISA with Human CD32b Human CD32a
產(chǎn)品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16b / FCGR3B (rh CD16b / FCGR3B, NP_000561.3, Met1- Ser200). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 FCGR3B
背景 CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide- anchored form (CD16a) in human natural killer cells and macrophages and as a GPI- anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.
NCBI Accession NP_000561.3

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Immunochemistry - IHC-P: 5-20 μg/mL
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
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