磷酸化細胞信號轉(zhuǎn)導分子SMAD2抗體

英文名稱 Phospho-Smad2(Ser245 + Ser250 + Ser255)
中文名稱 磷酸化細胞信號轉(zhuǎn)導分子SMAD2抗體
別    名 phospho-Smad2(p-SerSer245/250/255); p-Smad2(Ser245/250/255); phospho-Smad2(p-Ser245/250/255); hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.

說 明 書 0.1ml  
產(chǎn)品類型 磷酸化抗體 
研究領域 腫瘤  免疫學  信號轉(zhuǎn)導  細胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, 
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 58kDa
細胞定位 細胞核 細胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser245/250/255
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.xy-6828R    MAGEL2黑色素瘤抗原樣基因2抗體
xy-6826R    MAGEC1黑色素瘤相關(guān)抗原C1抗體
xy-6822R    MAGEB2黑色素瘤相關(guān)抗原B2抗體
xy-6824R    MAGEB4黑色素瘤相關(guān)抗原B4抗體
xy-6829R    MGEA5組蛋白乙酰轉(zhuǎn)移酶抗體（腦膜瘤表達抗原5）
xy-5468R    phospho-MAP4(Ser1073)磷酸化微管相關(guān)蛋白4抗體
xy-6823R    MAGEB3黑色素瘤相關(guān)抗原B3抗體
xy-7135R    MAZ鋅指蛋白801抗體
xy-10455R    NDUFS2NDUFS2抗體
xy-10456R    NDUFAF4NDUFAF4抗體
xy-1584R    NDRG1分化相關(guān)基因NDRG1抗體
xy-0072R    NTRH神經(jīng)降壓素抗體
xy-1420R    NCoR2核受體輔助抑制因子2抗體
xy-4757R    Nucleophosmin核仁磷酸蛋白抗體
xy-3616R    NCF4嗜中性粒細胞胞漿因子4抗體
xy-3485R    Phospho-NFKB p65(Ser468)磷酸化細胞核因子NF-κB p65抗體
xy-3731R    Phospho-eNOS (Thr495)磷酸化一氧化氮合成酶3（內(nèi)皮型）抗體
xy-3658R    NIPP1核抑制蛋白磷酸酶1抗體
xy-1466R    Nck1NCK銜接蛋白1抗體
xy-2408R    alpha Internexinα-中連蛋白抗體
xy-3563R    NR1D1細胞核受體Rev-Erbα抗體
xy-2420R    NCR2細胞毒性受體NK-p44抗體
xy-2416R    NKG2CNK細胞受體2C抗體
xy-2417R    NCR1細胞毒性受體NK-p46抗體
xy-2418R    NCR3細胞毒性受體NK-p30抗體
xy-3304R    Phospho-NMDAR2A (Tyr1246)磷酸化谷氨酸受體2A抗體
xy-0938R    NKG2DNK細胞受體2D抗體
xy-1342R    NRF-1核呼吸因子-1抗體
xy-0937R    NPY2R神經(jīng)肽Y受體2抗體