pVectOZ Transfection Plasmids are engineered in an optimized backbone and are ideal for all transfections. OZ Biosciences proposes five expressions vectors encoding for the most popular reporter genes (CAT, GFP, LacZ, Luciferase, SEAP)

pVectOZ- encoding genes:

Chloramphenicol Acetyltransferase (CAT) – pVectOZ-CAT

Green Fluorescent Protein (GFP) – pVectOZ-GFP

beta-Galactosidase (Lac Z) – pVectOZ-LACZ

Luciferase (Luc) – pVectOZ-LUC

Secreted Alcaline Phosphatase (SEAP) – pVectOZ-SEAP

The transgene expression level depends mainly on the promoter, enhancer, terminator and plasmid backbone. The pVectOZ expression cassette was designed to express transgene product in many mammalian cells and tissues. This vector has been modified to eliminate sequences affecting transgene expression levels while optimizing those critical for the high levels of expression. The final expression cassette accommodates the high levels of transgene expression in mammalian cells as well as high yield of plasmid production in Escherichia coli. The resulting plasmid is the ideal vector for expression in vitro and in vivo.

pVectOZ main features:

Highest levels of transgene expression in mammalian cells and tissues

Suitable for all transfection applications (in vivo & in vitro)

High copy number production in Escherichia coli

LPS-endotoxin free, supercoiled and highly purified: transfection grade approved

Sizes: 25µg or 100µg

Storage: -20°C

Shipping Conditions: Room Temperature

艾美捷OZ Biosciences pVectOZ轉(zhuǎn)染質(zhì)粒#PL00010在優(yōu)化的主鏈中設(shè)計(jì)，是所有轉(zhuǎn)染的理想選擇。OZ Biosciences提出了五種表達(dá)載體，編碼流行的報(bào)告基因（CAT，GFP，LacZ，Luciferase，SEAP）

pVectOZ-編碼基因：

氯霉素乙酰轉(zhuǎn)移酶 （CAT） – pVectOZ-CAT

綠色熒光蛋白 （GFP） – pVectOZ-GFP

β-半乳糖苷酶 （Lac Z） – pVectOZ-LACZ

熒光素酶 （Luc） – pVectOZ-LUC

分泌的堿性磷酸酶 （SEAP） – pVectOZ-SEAP

轉(zhuǎn)基因表達(dá)水平主要取決于啟動(dòng)子、增強(qiáng)子、終止子和質(zhì)粒骨架。pVectOZ表達(dá)盒設(shè)計(jì)用于在許多哺乳動(dòng)物細(xì)胞和組織中表達(dá)轉(zhuǎn)基因產(chǎn)物。該載體已經(jīng)過(guò)修改，以消除影響轉(zhuǎn)基因表達(dá)水平的序列，同時(shí)優(yōu)化那些對(duì)高表達(dá)水平至關(guān)重要的序列。最終的表達(dá)盒適應(yīng)哺乳動(dòng)物細(xì)胞中高水平的轉(zhuǎn)基因表達(dá)以及大腸桿菌中質(zhì)粒產(chǎn)生的高產(chǎn)量。所得質(zhì)粒是體外和體內(nèi)表達(dá)的理想載體。

OZ Biosciences pVectOZ轉(zhuǎn)染質(zhì)粒主要特點(diǎn)：

哺乳動(dòng)物細(xì)胞和組織中高水平的轉(zhuǎn)基因表達(dá)

適用于所有轉(zhuǎn)染應(yīng)用（體內(nèi)和體外）

大腸桿菌拷貝數(shù)高

不含LPS-內(nèi)毒素，超螺旋和高度純化：轉(zhuǎn)染級(jí)認(rèn)證

規(guī)格： 25μg 或 100μg

儲(chǔ)存： -20°C

運(yùn)輸條件：室溫

OZ Biosciences pVectOZ轉(zhuǎn)染質(zhì)粒的結(jié)果：

圖1：COS-7細(xì)胞中的熒光素酶劑量反應(yīng)。COS-7使用多量的pVectOZ-LUC和DreamFect™轉(zhuǎn)染試劑進(jìn)行轉(zhuǎn)染。轉(zhuǎn)染后48h測(cè)定熒光素酶活性。

圖2：MCF-7細(xì)胞中的X-Gal表達(dá)。MCF-7使用DreamFect Gold™轉(zhuǎn)染試劑轉(zhuǎn)染0.5μg pVectOZ-LacZ。使用X-Gal染色試劑盒（GX48）在轉(zhuǎn)染后10003h監(jiān)測(cè)β-半乳糖苷酶活性。