Amyjet SynaptoGreen C4 # 70020, a widely used green fluorescent dye, is used to track synaptic activity by staining synaptic vesicle at synapses or neuromuscular junctions. When combined with the red fluorescent dye SynaptoRed ™  When used in combination, synapses or neuromuscular junctions can be independently imaged in two colors. The research results of Tsien and Kay's laboratory indicate that when using SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) to reduce background staining, brain slices can be synaptoGreen ™ Staining.

SynaptoGreen C4 attribute:

For live or fixed cells: for live/intact cells

Detection type/option: Real time imaging

Color: Green

CAS number: 149838-22-2

Excitation/emission: 480/598 nm (in film)

Detection of cell localization: membrane/vesicle

Biotium provides a variety of nerve endings staining kits for staining brain slices. Please also refer to AM1-43 (70024), which is compatible with SynaptoGreen ™  Similar, but with amine groups that can immobilize formaldehyde.

SynaptoGreen ™  It can also be used to stain bacteria (Gram positive or Gram negative) and yeast, although it quickly internalizes into the vacuole membrane in yeast. For more information on how Biotium dyes stain various organisms, please refer to its cell staining table.

SynaptoGreen C4已用于標(biāo)記EM網(wǎng)格上生長(zhǎng)的培養(yǎng)神經(jīng)元，適用于熒光標(biāo)記、活體光學(xué)顯微鏡和成像研究等。

一種適合于在突觸和神經(jīng)肌肉接頭處監(jiān)測(cè)突觸活動(dòng)的熒光神經(jīng)末梢染料

SynaptoGreen C4注意事項(xiàng)：

保存和操作過(guò)程中注意避光

在水溶液中基本無(wú)熒光，最大發(fā)射波長(zhǎng)具pH依賴性。苯乙烯染料的光譜特征在甲醇或氯仿中測(cè)定。其在膜環(huán)境中的最大激發(fā)和最大發(fā)射波長(zhǎng)都會(huì)變短。最大激發(fā)波長(zhǎng)的差異通常在20nm，發(fā)射波長(zhǎng)的差異通常是80nm，但依具體的探針有所差異。

艾美捷SynaptoGreen C4#70020 ，一種廣泛使用的綠色熒光染料，用于通過(guò)對(duì)突觸或神經(jīng)肌肉接頭處的突觸小泡進(jìn)行染色來(lái)跟蹤突觸活動(dòng)。當(dāng)與紅色熒光染料 SynaptoRed™ ( 70021 ) 結(jié)合使用時(shí)，突觸或神經(jīng)肌肉接頭可以以兩種顏色獨(dú)立成像。Tsien 和 Kay 實(shí)驗(yàn)室的研究結(jié)果表明，當(dāng)使用 SR101（Sulforhodamine 101、80101）或 ADVASEP-7（70029）來(lái)減少背景染色時(shí)，腦切片可以被SynaptoGreen ™染色。

SynaptoGreen C4屬性：

對(duì)于活細(xì)胞或固定細(xì)胞：對(duì)于活細(xì)胞/完整細(xì)胞

檢測(cè)類型/選項(xiàng)：實(shí)時(shí)成像

顏色：綠

CAS號(hào)：149838-22-2

激發(fā)/發(fā)射：480/598 nm（在膜中）

探測(cè)細(xì)胞定位：膜/囊泡

Biotium 提供多種用于染色腦切片的神經(jīng)末梢染色試劑盒。另請(qǐng)參見(jiàn) AM1-43 ( 70024 )，它與 SynaptoGreen™ 類似，但具有可固定甲醛的胺基。

SynaptoGreen™ 也可用于對(duì)細(xì)菌（革蘭氏陽(yáng)性或革蘭氏陰性）和酵母進(jìn)行染色，盡管在酵母中它會(huì)迅速內(nèi)化到液泡膜中。有關(guān)Biotium的染料如何染色各種生物體的更多信息，請(qǐng)參閱它的細(xì)胞染色表 。