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Source

Rabbit

Applications

WB,IHC,ELISA

Reactivity

Human,Mouse,Rat,Monkey

Dilution

WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. ELISA: 1:5000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range:111-160

Uniprot No

P20963

Alternative names

CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

CD247 molecule(CD247) Homo sapiens The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Other

CD247, T-cell surface glycoprotein CD3 zeta chain

Mol.Wt (Da)

18696

Concentration

1 mg/ml