Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究
Exalpha公司簡(jiǎn)介
Exalpha有限公司于2000年在馬薩諸塞州雪莉（美國(guó)）成立。該公司主要通過(guò)開(kāi)發(fā)和生產(chǎn)抗體來(lái)支持生命科學(xué)領(lǐng)域的研究人員和科學(xué)家。
Exalpha公司產(chǎn)品領(lǐng)域
Exalpha公司其領(lǐng)域涉及信號(hào)通路、凋亡、增殖、炎癥、免疫學(xué)、傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究等方面。Exalpha主要產(chǎn)品線包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多個(gè)產(chǎn)品線，可提供給全球的科研實(shí)驗(yàn)室，制藥企業(yè)，生物技術(shù)和診斷公司，醫(yī)院等，目前旗下的代理公司遍布全球。
細(xì)胞增殖是生物體的重要生命特征，是生物體生長(zhǎng)、發(fā)育、繁殖以及遺傳的基礎(chǔ)。監(jiān)測(cè)細(xì)胞群的生長(zhǎng)對(duì)細(xì)胞的狀態(tài)研究很有必要?？蒲泄ぷ髡邆兘?jīng)常需要檢測(cè)細(xì)胞增殖情況，比如驗(yàn)證抗腫瘤藥物的藥效時(shí)，監(jiān)測(cè)細(xì)胞增殖及存活情況；研究腫瘤侵襲與轉(zhuǎn)移模型時(shí)，分析細(xì)胞轉(zhuǎn)移或侵襲的路徑；分析創(chuàng)傷的修復(fù)或組織的重建時(shí)，檢測(cè)細(xì)胞生長(zhǎng)情況等等。檢測(cè)細(xì)胞增殖及活性變化則可以很好的反映細(xì)胞的生長(zhǎng)狀況。
細(xì)胞增殖的研究方法
細(xì)胞增殖的研究方法有很多種，根據(jù)檢測(cè)原理不同，主要分為DNA合成檢測(cè)法、代謝活性檢測(cè)法、細(xì)胞增殖標(biāo)志物檢測(cè)法等。本文重點(diǎn)介紹DNA合成檢測(cè)之Brdu檢測(cè)法，傳統(tǒng)DNA合成檢測(cè)方法使用胸腺嘧啶核苷（3H-TdR）摻入DNA，然后通過(guò)放射自顯影或閃爍計(jì)數(shù)對(duì)DNA進(jìn)行定量，該方法由于存在放射性物質(zhì)、實(shí)驗(yàn)周期長(zhǎng)、操作繁瑣，且需要昂貴的設(shè)備，目前已被BrdU（5-Bromo-2-deoxyuridine，5-溴脫氧尿嘧啶核苷）檢測(cè)方法取代，BrdU作為一種胸苷類似物，可選擇性地?fù)饺朐鲋臣?xì)胞的DNA中，而且可穩(wěn)定存在，并帶到子代細(xì)胞中，細(xì)胞經(jīng)過(guò)固定和變性處理后，可用免疫學(xué)方法檢測(cè)DNA中BrdU的含量，從而判斷細(xì)胞的增殖能力。
Exalpha Biologicals品牌BrdU檢測(cè)試劑盒
因組織細(xì)胞內(nèi)無(wú)內(nèi)源性BrdU存在，可利用BrdU相關(guān)抗體來(lái)檢測(cè)和定位摻入活躍增殖細(xì)胞新合成DNA的BrdU，Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒的優(yōu)勢(shì)在于使用細(xì)胞層作為固相，將BrdU試劑摻入微孔板中的細(xì)胞，除了對(duì)細(xì)胞增殖情況進(jìn)行評(píng)估外，還可以從單個(gè)培養(yǎng)物中分析細(xì)胞數(shù)量、形態(tài)和細(xì)胞抗原等信息。同時(shí)Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒可通過(guò)試劑盒自帶的固定/變性溶液一步完成細(xì)胞固定、透化和DNA變性等實(shí)驗(yàn)流程，大大縮短了實(shí)驗(yàn)時(shí)間。Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒中包含檢測(cè)細(xì)胞增殖活性所需的所有組分、操作簡(jiǎn)便、無(wú)放射性、廣泛的樣本適用性，已得到眾多科研工作者的青睞。
Exalpha公司熱銷產(chǎn)品
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
BrdU Cell Proliferation Assay Kit | X1327K1 | 200Tests |
chx 10 (Visual system homeobox 2) (NT) Polyclonal Antibody | X1180P | 250ug |
EBNA 3C Polyclonal Antibody | F125P | 250ug |
SURF1 Polyclonal Antibody | X1562P | 100ug |
Bnip3L (IN) (nix) (bcl 2 family) Polyclonal Antibody | X1120P | 100ug |
chx 10 (Visual system homeobox 2) (CT) Polyclonal Antibody | X1179P | 250ug |
Microphthalmia Transcription Factor (MiTF) Monoclonal Antibody | X1405M | 100ug |
EBNA 3A Polyclonal Antibody | F115P | 250ug |
CD5 PE Single-Color Reagent | 0054 | 100Tests |
PTP mu Monoclonal Antibody | P100M | 100ug |
CD117 FITC Single-Color Reagent | 1172 | 100Tests |
Kv1.5 Potassium Channel Polyclonal Antibody | X1501P | 100ug |
N-SMase Activation Associated Factor Antigen Immunoaffinity Purified Polyclonal | X2354P | 100ug |
Chicken anti Glutathione S Transferase (GST), conjugated with HRP | AGST-HRP | 0.1 mg |
CD7 PE Single-Color Reagent | 0074 | 100Tests |
Protein Phosphatase 1 alpha Polyclonal Antibody | P105P | 100ug |
CD2 PE Single-Color Reagent | 0024 | 100Tests |
Kv1.6Potassium Channel Polyclonal Antibody | X1502P | 100ug |
Degenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity Purified Polyclonal | X2356P | 100ug |
CD8 PE Single-Color Reagent | 0084 | 100Tests |
Protein Phosphatase 1 beta Polyclonal Antibody | P110P | 100ug |
CD2 FITC - CD7 PE Bi-Test Reagents (FITC/RPE) | 0027S | 50Tests |
Kv2.1Potassium Channel Polyclonal Antibody | X1503p | 100ug |
Sphingomyelin Phosphodiesterase 3 Antigen Immunoaffinity Purified Polyclonal | X2359P | 100ug |
Chicken anti HA (Hemagglutinin) Epitope | AHA | 0.1 mg |
CD13 PE Single-Color Reagent | 0134 | 100Tests |
Protein Phosphatase 1 beta Polyclonal Antibody | P115P | 100ug |
CD3 PE Single-Color Reagent | 0034 | 100Tests |
Potassium Channel (Kv3.1b ) Polyclonal Antibody | X1504P | 100ug |
Monocarboxylate Transporter 3 (MCT3) Antigen Immunoaffinity Purified Polyclonal | X2361P | 100ug |
Protein Phosphatase 1 gamma 1 Polyclonal Antibody | P120P | 100ug |
CD61 PE Single-Color Reagent | 0614 | 100Tests |
Potassium Channel (Kv4.2 ) Polyclonal Antibody | X1505P | 100ug |
Zinc Finger Protein GLI1 Antigen Immunoaffinity Purified Polyclonal | X2363P | 100ug |
Chicken anti HA (Hemagglutinin) Epitope, conjugated with FITC | AHA-F | 0.1 mg |
CD16 PE Single-Color Reagent | 0164 | 100Tests |
Protein Phosphatase 1 gamma 1 Polyclonal Antibody | P125P | 100ug |
BrdU (bromodeoxyuridine) Monoclonal Antibody | X1028 | 100ug |
Calcium Channel (a1B) Polyclonal Antibody | X1507P | 100ug |
Monocarboxylate Transport 2 Antigen Immunoaffinity Purified Polyclonal | X2368P | 100ug |
Chicken anti HA (Hemagglutinin) Epitope, conjugated with HRP | AHA-HRP | 0.1 mg |
CD18 PE Single-Color Reagent | 0184 | 100Tests |
Protein Phosphatase 1 gamma 2 Polyclonal Antibody | P130P | 100ug |
Muc1 Monoclonal Antibody | X2783M | 100ug |
Voltage-dependent T-type calcium channel subunit alpha-1E Polyclonal Antibody | X1509P | 100ug |
G-Protein Coupled Receptor 94 Antigen Immunoaffinity Purified Polyclonal | X2373P | 100ug |
CD19 PE Single-Color Reagent | 0194 | 100Tests |
Protein Phosphatase 2 A/A Polyclonal Antibody | P140P | 100ug |
CD95 Monoclonal Antibody | X2785M | 100ug |
Calcium Channel (a1G) Polyclonal Antibody | X1510P | 100ug |
