- quickzyme是歐洲著名的EILISA 試劑盒提供商,為很多大的試劑公司OEM試劑盒,其產(chǎn)品高質(zhì)量和高靈敏性及穩(wěn)定性得到各大實(shí)驗(yàn)室及制藥公司認(rèn)可,其Granzyme B ELISA KIT 早已經(jīng)被世界各大實(shí)驗(yàn)室使用,高品質(zhì)的質(zhì)量得到眾多客戶的選擇
- bace1(β-site app cleaving enzyme),即β-分泌酶,是淀粉樣前體蛋白(amyloid precursor protein,app)產(chǎn)生aβ的關(guān)鍵酶。bace1屬于ⅰ型跨膜蛋白,由501個氨基酸組成,其中46~460位氨基酸為功能活性區(qū)域。bace1通過水解app670met-671asp之間的肽鍵,生成670個氨基酸的可溶性n末端和包含完整aβ區(qū)的app跨膜c端。后者經(jīng)γ分泌酶進(jìn)一步酶切形成aβ。zui近的研究表明,bace1基因敲除小鼠腦內(nèi)的aβ水平可降低90%,且其生理表型基本正常,顯示了bace1抑制劑在ad治療中的廣闊前景[1]。通過酵母或昆蟲表達(dá)系統(tǒng)表達(dá)活性bace1在zui近已見報道[2,3]。然而,由于上述系統(tǒng)表達(dá)的蛋白量低,且純化流程復(fù)雜,不適合bace1抑制劑的大規(guī)模篩選。而bace1的原核表達(dá)可克服以上缺陷,為篩選特異性抑制劑及研究bace1結(jié)構(gòu)與功能關(guān)系提供大量材料,為zui終從根本上治療ad帶來希望。
- Assay specifications
- Reagent set for five 96-well plates
- Measures active human Granzyme B
- Samples: conditioned cell culture media, cell extracts, body fluids (plasma, wound fluid ), tissue extracts, and (purified) enzyme preparations
- Quantitative
- Range/sensitivity: 0.01 ng/ml (4 h incubation)
- Ease-of-use: Equivalent to ELISA
- Overnight protocol
- Store at 20°C for long term storage
- Reagent set contains antibody, standard, detection enzyme and substrate
This kit is developed by QuickZyme BioSciences and is available via Sigma Aldrich.