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訪問次數(shù):527更新時(shí)間:2021-09-06 17:11:30
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供貨周期 | 現(xiàn)貨 | 規(guī)格 | 1ug |
---|---|---|---|
貨號(hào) | 11509ES03 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè) |
產(chǎn)品信息
產(chǎn)品名稱 | 產(chǎn)品編號(hào) | 規(guī)格 |
RBP-JK luciferase reporter plasmid (RBP-JK-Luc螢光素酶報(bào)告基因質(zhì)粒) | 11509ES03 | 1 μg |
產(chǎn)品描述
RBP-JK-Luc螢光素酶報(bào)告基因(報(bào)告基因質(zhì)粒)(RBP-JK luciferase reporter plasmid)是翌圣生物自主研發(fā)的用于檢測(cè)RBP-JK轉(zhuǎn)錄活性水平為目的的報(bào)告基因。RBP-JK蛋白(CSL/CBF1/Su (H)/Lag1)是一種對(duì)Notch信號(hào)通路下游進(jìn)行調(diào)控的轉(zhuǎn)錄因子。
RBP-JK報(bào)告基因主要用于檢測(cè)細(xì)胞中Notch信號(hào)通路的活性、藥物研究以及基因過表達(dá)和RNAi的表型分析等。
pRBP-JK-Luc是翌圣生物改造后的哺乳動(dòng)物真核表達(dá)載體,在其多克隆位點(diǎn)插入了多個(gè)RBP-JK結(jié)合位點(diǎn),可以高靈敏度地檢測(cè)RBP-JK的激活水平。同時(shí),對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?,增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小,使得RBP-JK報(bào)告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜
載體元件信息
RBP-JK response element (RBP-JK) | 32-92 |
Minimal TA promoter (pTA) | 120-142 |
Luciferase reporter gene | 184-1836 |
SV40 late poly(A) signal | 1880-2001 |
SV40 early promoter | 2195-2552 |
Synthetic neomycin phosphotransferase(Neor) coding region | 2583-3377 |
Synthetic poly(A) signal | 3402-3450 |
Synthetic Beta-lactamase(Ampr) coding region | 4565-5425 |
Synthetic poly(A) signal/transcriptional pause site | 5530-5683 |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。保質(zhì)期1年。
注意事項(xiàng)
1)本質(zhì)粒未經(jīng)翌圣生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2)為了您的健康,實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和戴一次性手套。
3)本產(chǎn)品僅作科研用途!
使用說明
pRBP-JK-Luc可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用螢光素酶檢測(cè)試劑盒或雙螢光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
參考文獻(xiàn)
[1] Hahm E R, Chandra-Kuntal K, Desai D, et al. Notch activation is dispensable for D, L-sulforaphane-mediated inhibition of human prostate cancer cell migration[J]. PloS one, 2012, 7(9): e44957.
[2] Lee J, Sehrawat A, Singh S V. Withaferin A causes activation of Notch2 and Notch4 in human breast cancer cells[J]. Breast cancer research and treatment, 2012, 136(1): 45-56.
[3] Kim S H, Sehrawat A, Singh S V. Notch2 activation by benzyl isothiocyanate impedes its inhibitory effect on breast cancer cell migration[J]. Breast cancer research and treatment, 2012, 134(3): 1067-1079.
[4] Guo S, Gonzalez-Perez R R. Notch, IL-1 and leptin crosstalk outcome (NILCO) is critical for leptin-induced proliferation, migration and VEGF/VEGFR-2 expression in breast cancer[J]. PloS one, 2011, 6(6): e21467.
[5] Wang R, Cheng L, Yang X, et al. Histone methyltransferase SUV39H2 regulates cell growth and chemosensitivity in glioma via regulation of hedgehog signaling[J]. Cancer cell international, 2019, 19(1): 1-12.
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