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- 11550ES03 產(chǎn)品型號
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供貨周期 | 現(xiàn)貨 | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
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Rb Luciferase Reporter Plasmid
(Rb-Luc熒光素酶報告基因質(zhì)粒)
產(chǎn)品信息
產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 | 儲存 | 價格(元) |
Rb luciferase reporter plasmid (Rb-Luc熒光素酶報告基因質(zhì)粒) | 11550ES03 | 1μg | -20oC | 3385.00
|
產(chǎn)品描述
Rb-Luc熒光素酶報告基因(報告基因質(zhì)粒)(Rb luciferase reporter plasmid)是翊圣生物自主研發(fā)的用于檢測Retinoblastoma protein轉(zhuǎn)錄活性水平為目的的報告基因。Retinoblastoma protein(Rb)是一種腫瘤抑制蛋白,在多種癌細(xì)胞中是發(fā)生功能障礙的。Rb是通過抑制處于分裂期細(xì)胞的細(xì)胞周期進(jìn)程阻礙細(xì)胞的過度增殖。
Rb報告基因主要用于檢測細(xì)胞中cell cycle信號通路活性、藥物研究以及基因過表達(dá)和RNAi的表型分析等。
pGMRb-Lu是翊圣生物改造后的哺乳動物真核表達(dá)載體,在其多克隆位點插入了多個Rb結(jié)合位點,可以高靈敏度地檢測Rb的激活水平。同時,對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點進(jìn)行了適當(dāng)?shù)耐蛔?,增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小,使得Rb報告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜
運輸與保存方法
冰袋(wet ice)運輸。收到產(chǎn)品后放到-20oC保存。
注意事項
本質(zhì)粒未經(jīng)翊圣生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實驗室以外的任何人或單位。
為了您的健康,實驗操作時請穿實驗服和帶一次性手套。
使用說明
pGMRb-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動物細(xì)胞。用熒光素酶檢測試劑盒或雙熒光素酶檢測試劑盒進(jìn)行檢測。
參考文獻(xiàn)
[1] Tsang WP ,et al. Oncofetal H19-derived miR-675 regulates tumor suppressor RB in human colorectal cancer. Carcinogenesis. 2010 Mar;31(3):350-8.
[2] Niesen MI ,et al. Technological advances in the study of HLA-DRA promoter regulation: extending the functions of CIITA, Oct-1, Rb, and RFX. ActaBiochimBiophys Sin (Shanghai). 2009 Mar;41(3):198-205.
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[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).
[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B. PLoS One. 6(8):e23660 (2011).
[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716:169-77 (2011).
[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).
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