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產(chǎn)品簡介
在細(xì)胞培養(yǎng)中，葡聚糖硫酸鈉5,000可通過改變細(xì)胞表面的電荷，來促使單個(gè)細(xì)胞懸浮，防止細(xì)胞結(jié)團(tuán)。在動(dòng)物實(shí)驗(yàn)中，已有多篇文獻(xiàn)驗(yàn)證，可用于小鼠等動(dòng)物結(jié)腸炎模型的誘導(dǎo)。本品有生化級(jí)別與分子生物學(xué)級(jí)別兩種可供選擇。
詳細(xì)介紹
葡聚糖硫酸鈉5,000
Sodium Dextran Sulfate 5,000
規(guī)格含量 : Total sulfur : 15.0～20.0%
生產(chǎn)商：FUJIFILM Wako Pure Chemical Corporation
保存條件 : 室溫
CAS RN® : 9011-18-1
分子量 : 1,000~9,000
◆產(chǎn)品概況
葡聚糖硫酸鈉，是一種由葡萄糖聚合，經(jīng)硫酸化后所得的化合物。本產(chǎn)品的平均分子量約為5000（分子量范圍：1000-9000），是聚陰離子物質(zhì)。在細(xì)胞培養(yǎng)中，本品可通過改變細(xì)胞表面的電荷，來促使單個(gè)細(xì)胞懸浮，防止細(xì)胞結(jié)團(tuán)。在動(dòng)物實(shí)驗(yàn)中，已有多篇文獻(xiàn)驗(yàn)證，可用于小鼠等動(dòng)物結(jié)腸炎模型的誘導(dǎo)。
本品有生化級(jí)別與分子生物學(xué)級(jí)別兩種可供選擇。生化級(jí)別為適用于生化實(shí)驗(yàn)的產(chǎn)品，尤其適用于β-脂蛋白的定量分析。分子生物學(xué)級(jí)別適用于核酸雜交等分子生物學(xué)實(shí)驗(yàn)，經(jīng)電泳或熒光法對(duì)DNase 和 RNase 的活性進(jìn)行檢測(cè)。聯(lián)系客服可獲取相應(yīng)產(chǎn)品規(guī)格書。
◆物性信息
外觀：白色~微淡黃色，結(jié)晶粉末~粉末
溶解性：易溶于水，幾乎不溶于乙醇和丙酮
pH信息：pH (50 g/L, 25℃) : 4.5～8
包裝：氬氣封裝
◆用途
1. 誘導(dǎo)小鼠、大鼠等動(dòng)物的結(jié)腸炎模型[1-12]；
2. 誘導(dǎo)小鼠的結(jié)腸癌模型[13]；
3. β-脂蛋白的定量分析中選擇性分離脂蛋白[14]；
4. 抑制人免疫缺陷病毒（HIV）的體外復(fù)制[15]；
5. 細(xì)胞培養(yǎng)中防止細(xì)胞結(jié)團(tuán)；
6. 促進(jìn)核酸雜交。
◆產(chǎn)品列表
產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品等級(jí) | 產(chǎn)品規(guī)格 |
197-08362 | Sodium Dextran Sulfate 5,000 葡聚糖硫酸鈉5,000 | for Biochemistry | 25 g |
199-08361 | 100 g | ||
| 191-08365 | 500 g | ||
| 194-13402 | for Molecular Biology | 25 g | |
| 196-13401 | 100 g | ||
| 198-13405 | 500 g |
※ 本頁面產(chǎn)品僅供研究用，研究以外不可使用。
參考文獻(xiàn)
1. | Hori, Y. , Hoshino, J. , Yamazaki, C. , Sekiguchi, T. , Miyauchi, S. , & Mizuno, S. , et al. (1997). Effect of lecithinized-superoxide dismutase on the rat colitis model induced by dextran sulfate sodium. Jpn J Pharmacol, 74(1), 99-103. DSS 5000溶解于蒸餾水中并制備成3%溶液（25mg/5ml/Kg）【IF=1.305，誘導(dǎo)大鼠結(jié)腸炎模型】 |
2. | Kawabata, K., Kanmura, S., Morinaga, Y., Tanaka, A., Makino, T., Fujita, T. ... Ido, A. (2019). A high?fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium?induced colitis. International Journal of Molecular Medicine, 43, 1487-1496. 全文 DSS 5000溶解于蒸餾水中并制備成1%溶液【IF=3.098，誘導(dǎo)小鼠結(jié)腸炎模型】 |
3. | Numata, Y. , Tazuma, S. , Nishioka, T. , Ueno, Y. , & Chayama, K. . (2010). Immune response in mouse experimental cholangitis associated with colitis induced by dextran sulfate sodium. Journal of Gastroenterology & Hepatology, 19(8), 910-915. DSS 5000溶解于蒸餾水中并制備成2.5%溶液【IF=1.83，誘導(dǎo)小鼠結(jié)腸炎模型】 |
4. | Ogawa, A., Andoh, Araki, Y., & Bamba, et al. (2004). Neutralization of interleukin-17 aggravates dextran sulfate sodium-induced colitis in mice. CLINICAL IMMUNOLOGY, 110(1), 55-62. 向小鼠喂食含有2.0%(wt/wt) DSS 5000的飼料【IF=3.368，誘導(dǎo)小鼠結(jié)腸炎模型】 |
5. | Nishimura, T. , Andoh, A. , Hashimoto, T. , Kobori, A. , Tsujikawa, T. , & Fujiyama, Y. . (2010). Cellobiose prevents the development of dextran sulfate sodium (dss)-induced experimental colitis. Journal of Clinical Biochemistry and Nutrition, 46(2). 向小鼠喂食含有3.5%(wt/wt) DSS 5000的飼料【IF=2.405，誘導(dǎo)小鼠結(jié)腸炎模型】 |
6. | Deguchi, Y., Andoh, A., Inatomi, O., Yagi, Y., Bamba, S., Araki, Y., Hata, K., Tsujikawa, T., & Fujiyama, Y. (2007). Curcumin prevents the development of dextran sulfate Sodium (DSS)-induced experimental colitis. Digestive diseases and sciences, 52(11), 2993–2998. 全文 向小鼠喂食含有3.5%(wt/wt) DSS 5000的飼料【IF=2.751，誘導(dǎo)小鼠結(jié)腸炎模型】 |
7. | Araki, Y., Mukaisyo, K., Sugihara, H., Fujiyama, Y., & Hattori, T. (2010). Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice . Oncology Reports, 24, 869-874. 全文 向小鼠喂食含有5%(wt/wt) DSS 5000的飼料【IF=3.417，誘導(dǎo)小鼠結(jié)腸炎模型】 |
8. | Katada, K., Yoshida,N., Suzuki,T., et al. (2008). Dextran sulfate sodium-induced acute colonic inflammation in angiotensin ii type 1a receptor deficient mice. Inflammation Research, 57(2), 84-91. 用飲用水向小鼠喂食3.0 % (w/vol) DSS 5000，持續(xù)7天【IF=3.174，誘導(dǎo)小鼠結(jié)腸炎模型】 |
9. | Iwanaga, T. , Hoshi, O. , Han, H. , & Fujita, T. . (1994). Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: some possible mechanisms of cecal ulceration. Journal of Gastroenterology, 29(4), 430. 向豚鼠喂食3.0 % DSS 5000水溶液【IF=6.132，誘導(dǎo)豚鼠結(jié)腸炎模型】 |
10. | Shintani, N. , Nakajima, T. , Okamoto, T. , Kondo, T. , Nakamura, N. , & Mayumi, T. . (1998). Involvement of cd4+ t cells in the development of dextran sulfate sodium-induced experimental colitis and suppressive effect of igg on their action. General Pharmacology: The Vascular System, 31(3), 477-481. 向小鼠喂食含有5.0 % (w/v) DSS 5000的飲用水，持續(xù)7天【IF=1.134，誘導(dǎo)小鼠結(jié)腸炎模型】 |
11. | Kanauchi, O. , Nakamura, T. , Agata, K. , Mitsuyama, K. , & Iwanaga, T. . (1998). Effects of germinated barley foodstuff on dextran sulfate sodium-induced colitis in rats. Journal of Gastroenterology, 33(2), 179-188. 向大鼠喂食含有3.0%(wt/wt) DSS 5000的飼料【IF=6.132，誘導(dǎo)大鼠結(jié)腸炎模型】 |
12. | Kao, N. J. , Hu, J. Y. , Wu, C. S. , & Kong, Z. L. . (2016). Curcumin represses the activity of inhibitor-κb kinase in dextran sulfate sodium-induced colitis by s-nitrosylation. International immunopharmacology, 38, 1-7. 向小鼠喂食含有5.0 % (w/v) DSS 5000的飲用水【IF=3.943，誘導(dǎo)小鼠結(jié)腸炎模型】 |
13. | Gu, S. , Papadopoulou, N. , Gehring, E. M. , Nasir, O. , Dimas, K. , & Bhavsar, S. K. , et al. (2009). Functional membrane androgen receptors in colon tumors trigger pro-apoptotic responses in vitro and reduce drastically tumor incidence in vivo. Molecular Cancer, 8(1), 114. 向小鼠喂食30g/L（即3.0%）的 DSS 5000水溶液，持續(xù)7天【IF=15.302，誘導(dǎo)小鼠結(jié)腸癌模型】 |
14. | Sakagami, T. , & Zilversmit, D. B. . (1961). Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis. Journal of Lipid Research, 2(3), 271-277. 向每毫升血清中加入0.04 ml的5％葡聚糖硫酸鹽和0.1 mL的11％氯化鈣。在4℃下靜置2小時(shí)后，將混合物以4,000 X g的速度離心10分鐘，去除上清液【IF=4.483，選擇性分離脂蛋白】 |
15. | Ito, M. , Baba, M. , Sato, A. , Pauwels, R. , Clercq, E. D. , & Shigeta, S. . (1987). Inhibitory effect of dextran sulfate and heparin on the replication of human immunodeficiency virus (hiv) in vitro.Antiviral Research , 7(6), 361-367. 使用PBS溶解（保存于4℃待用）【IF=4.101，抑制人免疫缺陷病毒（HIV）的體外復(fù)制】 |
16. | Yoshio, A., kira, A., Yoshihide, F., Kazunori, H.,Jin, M., Takafumi, O., Fumiyasu, N., Tadao, B., et al. (2001). Application of 2-aminopyridine fluorescence labeling in the analysis of in vivo and in vitro metabolism of dextran sulfate sodium by size-exclusion high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 753( 2), 209-215. 0.2mol的 DSS 5000溶解于25mL的氨基吡啶溶液中【利用HPLC分析在體內(nèi)外的代謝】 |